Protein microarray synthesis and label-free interaction analysis
Du sparer
0%
ift. normalprisen
Spar
0%
- Indbinding:
- Paperback
- Sideantal:
- 196
- Udgivet:
- 13. marts 2018
- Størrelse:
- 150x13x220 mm.
- Vægt:
- 310 g.
- 2-3 uger.
- 16. december 2024
På lager
Forlænget returret til d. 31. januar 2025
Normalpris
Abonnementspris
- Rabat på køb af fysiske bøger
- 1 valgfrit digitalt ugeblad
- 20 timers lytning og læsning
- Adgang til 70.000+ titler
- Ingen binding
Abonnementet koster 75 kr./md.
Ingen binding og kan opsiges når som helst.
- 1 valgfrit digitalt ugeblad
- 20 timers lytning og læsning
- Adgang til 70.000+ titler
- Ingen binding
Abonnementet koster 75 kr./md.
Ingen binding og kan opsiges når som helst.
Beskrivelse af Protein microarray synthesis and label-free interaction analysis
Protein microarrays are essential tools for high-throughput proteome interaction and binding kinetics analysis. Recent technical developments have resulted in approaches for the generation of protein arrays by in situ cell-free expression of DNA array templates. We combined and enhanced these ideas by creating innovative microfluidic flow cells in standard microscope slide format and such improved system efficiency, operability, robustness and controllability. An automated setup suitable for both the in situ expression as well as the interaction analysis of protein microarrays by flow-injection was designed, built and evaluated. The reflecto-interferometric principle was selected to enable high-throughput label-free imaging detection. Both, the protein microarray expression and the microarray interaction analysis could be performed sequentially in the same microfluidic flow cell - the created proteins remain in their fluidic environment and never get in contact with air. Various antibody-antigene and rabbit-sera immunization assays were proving this system to be an efficient tool for interaction screenings.
Brugerbedømmelser af Protein microarray synthesis and label-free interaction analysis
Giv din bedømmelse
For at bedømme denne bog, skal du være logget ind.Andre købte også..
Find lignende bøger
Bogen Protein microarray synthesis and label-free interaction analysis findes i følgende kategorier:
© 2024 Pling BØGER Registered company number: DK43351621