Method Development in Analytical HPLC

Method Development in Analytical HPLC presents the essential information for understanding the process of developing an HPLC method of analysis. It includes foundational information related to HPLC, as well as discussion of sample types, the properties of analytes and matrices in the samples, and sample preparation, which is an important step in analytical methods development. The book provides analytical methods from literature, informs development of original methods with selection of the suitable type of HPLC, of specific columns, mobile phase, and detection techniques with emphasis on the use of mass spectrometry for detection, as well as optimization and validation methods. In addition, it includes useful details on method development for specific types of chromatography, such as RP-HPLC, HILIC, ion exchange, size exclusion, chiral, and information on green chemistry in analytical methods, computer assisted method development, and other key contemporary aspects of the subject.