Bøger udgivet af Springer International Publishing

This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using confocal microscopy; evaluation of connexin HC activity in vivo; generation of connexin-expressing stable cell pools; and automated assessment of cutaneous pathology in a mouse model of HC dysfunction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Connexin Hemichannels: Methods and Protocols is a valuable resource that discusses the importance of HCs as drug targets, and how studying them is essential to identifying newcompounds capable of modulating HC function selectively and specifically.

This second edition provides new and updated methods for establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, that would be critical in exploring novel discoveries in cardiac biology, and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Experimental Models of Cardiovascular Diseases: Methods and Protocols, Second Edition aims to provide detailed and practical protocols that will be valuable tools for researchers in cardiology to conduct their research.

This fully updated volume explores N-Methyl-D-aspartate receptors (NMDARs), and the structure-function relations, principles, and rules that govern how NMDARs operate in brain processing under normal and pathological conditions. With chapters from leading laboratories around the world, this book examines the expression and purification of diverse NMDA receptor subtypes, gene targeting and generation of conditional NMDAR mutant mouse lines, studies of NMDARs in zebrafish, electrophysiological studies, NMDARs modeling in silico, drug development with artificial intelligence, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, NMDA Receptors: Methods and Protocols, SecondEdition serves as an ideal guide to the diversity of possible approaches in the field of NMDARs and the progress that has been made in recent years.

This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Imaging Cell Signaling is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.

This second edition provides new and updated chapters covering computational and mathematical techniques and concepts related to the field of comparative genomics. The topics covered in the chapters range from those that address general techniques and concepts that apply to all organisms to others that are specialized and apply to specific biological systems such as viruses, bacteria, nematodes, and insects. Well-known comparative genomics web-based platforms are also covered in specific chapters. Written in the highly successful Methods in Molecular Biology series format, many chapters include introductions to their respective topics and step-by-step comparison procedures, demonstrated on actual sets of genome sequences.Authoritative and cutting-edge, Comparative Genomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

This comprehensive volume provides balanced and easily readable chapters on contemporary topics around discourse production in patients with neurogenic disorders. Discourse broadly refers to a unit of language longer than a single sentence, typically used in a spoken or written format to express ideas, feelings, and opinions or to interact with others. As the field of research in speech-language pathology, aphasia and related neurogenic disorders, and clinical decision-making to manage neurogenic impairments is rapidly expanding, there has been an increased focus on discourse analysis and therapy to enable patients to speak well again. Various user groups and people with lived experience (e.g., aphasia, dementia, traumatic brain injury, right hemisphere damage, etc.) are also increasingly focused on the impact of neurogenic impairments on domains of conversation and changes in language skills beyond the smaller linguistic components of single words and sentences that are traditionally supported by speech-language therapy. The first part of this book details and discusses the basic principles, historical perspectives (history and developmental milestones), neural basis, and recent evolutions and advancement of discourse analysis in speakers with acquired communication disorders. The second part of the book outlines and illustrates current methods for discourse elicitation and analysis across different clinical populations. Recent leaps forward in technology that can assist in the recording, processing, and analysis of discourse will be highlighted. The final part of book reviews and summarizes key developments of various evidenced-based training strategies, interventions, and treatment programs, as well as utilization of innovative technology to clinically improve neurogenic disordered discourse.¿This is an ideal guide for researchers, clinicians, and students in the field of communication sciences and disorders, speech and language therapy, gerontology, (neuro-)linguistics, psychology, and related fields in clinical medicine.

Bringing together an international set of scholars, this volume presents integrative theoretical and methodological perspectives linking two complementary approaches in anthropological archaeology: cultural landscapes and human ecology. Authors grapple with issues ranging from the hunter-gatherer populations of North America and the emergence of the Neolithic in Europe to contemporary hunter-gatherer societies, using approaches from ethnoarchaeology to geomorphology, and methodological specialties from stable isotopes to social networks, in order to shed light on prehistoric human adaptations and how they produce cultural variation on a landscape scale. Together, contributions to this volume illustrate how interdisciplinary and integrative perspectives can aid archaeology by providing the means necessary to interpret and explain long-term records of human activity.This book capitalizes on the unique position of archaeology, and the long-term records of human ecology and cultural resilience the discipline develops, to make significant contributions to contemporary discussions of long-term climate human-environment interactions throughout the Holocene. The book is therefore produced during a perfect time in which other disciplines are focusing on the unique contribution that can be made by archaeology.

This book is an interdisciplinary work that reveals the contemporary sustainability narratives in India within the context of various SDGs. Several case studies are presented, each of which sheds light on the challenges and constraints that are impeding the process of achieving SDGs and investigates potential long-term answers to socio-economic and environmental issues. Through empirical case studies from different parts of India, the book explores the current status of achieving sustainable development goals in India. The volume immensely benefits scientists, researchers, policymakers and practitioners as it offers a thematic and comprehensive understanding of challenges associated with mainstreaming SDGs at national, sub-national, and micro scales in India.

Sustainable Engineering: Concepts and Practices provides insights into current perspectives on sustainable engineering research. It highlights the drivers, motivations, and challenges affecting the development and adoption of sustainable engineering in various sectors of the economy and how they impact sustainable development. Contributions from researchers representing multiple branches of engineering in academia, government laboratories, and industry present alternative approaches to traditional engineering practices. These approaches effect change, making the design, construction, production, and management of products, processes, and systems more environmentally friendly, socially beneficial, and economically profitable. The book will be a trusted reference for graduate students, practicing engineers, and other professionals interested in developing or using sustainable products and systems.

The volume is part of World Geomorphological Landscapes series and shows characteristic landscapes and landforms of Poland. It consists of two parts, general and regional. The first part includes overviews of geological history, Quaternary, climate and its impact on landforms, geomorphological division of the country, geoheritage and geoconservation. The second part comprises more nearly 40 region-specific studies to show the diversity of landscapes, from high-mountain relief through medium-altitude forested ranges, limestone uplands and loess plateaus, lowland landscapes moulded by past ice sheets, rivers and aeolian processes, to coastal sceneries. Selected examples of anthropogenically modified landscapes are also considered. Each chapter is accompanied by photographs, maps and diagrams. The volume is intended primarily for geographers and Quaternary geologists, but will be also of interest to anyone interested in natural landscapes and their conservation.

Sustainability is one of the most critical challenges facing humanity. Changing Higher Education Institutions (HEIs) is crucial to overcoming that challenge because of their roles providing cutting edge knowledge and preparing our future citizens, workforce, and thought leaders. Many educational organizations are already leading the way toward sustainability, but a multitude of others still have substantial work to do. The field of Industrial/Organizational (I/O) psychology offers a wealth of knowledge about human behavior within organizations that can support the changes essential for moving toward sustainability. This book serves as a one-stop-shop for individuals intent on engaging their HEIs in sustainability, but who have little experience with organizational change or have encountered barriers to progress. It synthesizes the empirical literature and describes through accessible prose the psychological principles to encourage structural, procedural, social, and behavioral changes in support of a socially just and environmentally sustainable future.

This book examines how enlightened Scottish social theorists c.1740 to c.1800 understood the origin and development of religion. Challenging scholarly disregard for the topic, it shows how most prominent thinkers of the Scottish Enlightenment thought deeply about the relationship between religion, human nature and historical change. The Scots viewed this relationship as an important strand within the study of the 'science of human nature' and the 'history of man.' The fruits of this investigation were a sophisticated and innovative account of religious change that is characterized by a striking modernity and naturalism, even by the more devout theorists. The views of the literati surveyed here need to be incorporated into our larger histories of the 'science of religion' as much as they do into our understanding of the social theory of the Scottish Enlightenment.

This comprehensive volume covers all aspects from the historical discovery of the filarial parasite Loa loa to the latest diagnostics and therapies for all forms of pathology used at the point of care. Current developments in these areas are also highlighted. Expert authors present the epidemiological impact of loiasis and the basic mechanisms underlying the disease in native populations and nonresidents of endemic areas. In addition to particulars on the immunology of the parasite, its general and molecular biology, the clinical manifestation is also described in detail. Both common and atypical clinical manifestations are considered. This book concludes with an outlook on further research and targeted eradication of the disease.Loa loa, known as the "eye worm", is endemic in the rural populations of several African countries and is transmitted by rainforest deerflies. It displays impressive immune adaptation with a wide range of clinical symptoms that remain largely unexplored. The characteristics of this nematode make it of great interest to physicians and researchers specializing in infectious diseases, epidemiology, and immunology. In addition, students and family physicians working with African immigrants and travelers should know about this parasite.Loiasis is a prime example of a neglected disease under the conditions of international health efforts in our globalized world. The present work is intended to promote progress in this field, making it a valuable read within the scope of the UN Sustainable Development Goal 3 (Good Health and Well-Being).

This book examines the social entrepreneurship strategies of nonprofit organizations (NPOs), with a focus on the Caribbean social sector. In addressing the conceptual ambiguities from an academic and experiential perspective, it aims to provide a much-needed reflection on social entrepreneurship (SE), including in developing contexts. Through a comparative analysis of the experiences of NPOs from the Caribbean, the authors demonstrate the applicability of SE for NPO sustainability and as an opportunity for social sector performance improvement. Blending both quantitative and qualitative methodologies, this work is a useful base for researchers wanting to advance the mission of theory and methodological development toward maturing the field of social entrepreneurship.

This book discusses the issue of riverbank erosion and its associated causes, risks and social challenges in the Bengal Delta. The book covers the physical processes and indicators of riverbank erosion such as sediment flux and channel morphology, the anthropogenic and natural causes of erosion, the social consequences that afflict vulnerable communities who depend on the delta for their livelihoods, and potential solutions to harmful erosion processes such as bioengineering methods and increased community governance through a socio-hydrological approach. Though the Bengal Delta is one of the most populated regions in the world, few publications exist that discuss riverbank erosion as a major threat to environmental and livelihood sustainability in a comprehensive manner. The book will fill this gap in research for students and researchers in geography and environmental sciences, and will engage regional planners, decision makers and policy makers to further their understanding of the complex social and environmental aspects of the Bengal Delta associated with persistent and worsening riverbank erosion.

At a time of widespread disillusion, citizens keep telling us how ¿frustrated¿ they feel with their democracies. However, whilst scholars and commentators alike have heard that complain millions of times, we may not have taken it as seriously as we should. The author takes the concept of democratic frustration literally and puts it under an unprecedented analytical and empirical microscope. She applies insights from the psychology and political science literatures and uses a mixture of panel studies, surveys, interviews, and experiments to understand its sources, nature, dimensions, and consequences. The book sheds unprecedented light on pathologies of democratic frustration in the US, UK, Australia, and South Africa with a double focus on the general population, and on young people. Doing so, it reveals new thought-provoking insights on the true nature of contemporary democratic crises, and not least on how citizens¿ actual desire for democracy uniquely shapes their dissatisfaction.