Bøger udgivet af Springer International Publishing

This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Thermomorphogenesis: Methods and Protocols serves as an ideal guide to this vital subject for research laboratories, as well as for graduate and undergraduate laboratory courses in plant molecular biology.

This volume explores the latest developments in the study of the cerebral cortex. The chapters in this book cover a wide array or established methods such as in situ hybridization of brain slices; two-photon FRET/FLIM imaging of cerebral neurons; BioID analysis of actin-binding proteins; generation of iPS cells using Sendai Virus vectors; in vivo whole-cell recording from the mouse brain; and locomotor assays in Drosphila larvae and adult flies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Cerebral Cortex Development: Methods and Protocols is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important field.

This volume provides protocols on different combinations of contaminates, matrices, and sample preparation. Chapters are divided into two parts, detailing polycyclic aromatic hydrocarbon,dioxins, furans, organochlorie pesticides, toxic elements, mycotoxins, mercury in food products, acrylamine, polypeptide antibiotics, tetracyclines, coccidiostats, beta-blockers, sedatives,glucocorticoids, palytoxin-like marine biotoxins in fish, polar drugs and contaminants in animal feed, UV filters, micro and nanoplastics in seafood, tetracyclines in vegetables, MCPDEs, and pharmaceuticals in seafood. Written in the format of the Methods and Protocols in Food Science (MeFS) series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readilyreproducible laboratory protocols, and contain notes on how to avoid or solve typical problems. Authoritative and cutting-edge,Chemical Food Contaminants Analysis aims to ensure successful results in the further study of this vital field

This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This fully updated edition explores fundamental protocols for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Featuring protocols to examine light response curves and gas exchange measurements for a variety of samples, the book includes new protocols on photosynthesis in the adaxial and abaxial sides of a leaf, non-foliar organs, and aquatic systems. Protocols and best practice for eddy covariance, thermal, spectral, and hyperspectral imaging and data analysis, as well as stable isotope labeling and quantification of photosynthetic metabolites, are introduced alongside fresh insights on many more topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Photosynthesis: Methods and Protocols, Second Edition provides an ideal guide to the improved technologies expanding our capabilities to study photosynthesis today.

This detailed volume describes the most recent methodology for buckwheat research. Buckwheat serves as a promising functional food source containing various phenolic compounds, such as rutin, quercetin, and C-glycosylflavones, which have a positive therapeutic or dietary effect for promoting human health. This book explores techniques for studying this pseudocereal, namely the two most widely cultivated species, F. esculentum (common buckwheat) and F. tataricum (Tartary buckwheat). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensible, Buckwheat: Methods and Protocols is an ideal guide for plant scientists working in this field.

This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understandingof this important and evolving field.

Ministerial Leadership offers a practice-based account of how ministers in UK governments perform their roles and exercise leadership in their spaces of activity. Drawing on the unique Ministers Reflect archive of the Institute for Government, which is an open and growing resource of over 140 ministerial interviews at UK and devolved government levels, as well as other ministerial reflections, the book addresses the literature on ministerial life and political leadership, and develops new concepts for examining ministerial leadership in different spheres. It argues that the relationship between ministers and civil servants has changed significantly in recent decades, as ministers place greater emphasis on delivery and implementation. The book adopts a theoretically pluralist approach with the intention of offering a valuable teaching aid for existing and new courses. It will appeal to all those interested in public policy and governance.

Pollen is made up of many chemical constituents such as proteins, sugars, lipids, minerals and phytochemicals. These components make pollen products an excellent source of nutrients and antioxidants for consumers. However, mycotoxigenic fungi are also common in pollen, creating contamination from generated mycotoxins. With adequate monitoring, sampling, processing and storage of pollen the development of mycotoxins can be significantly prevented. Since pollen grain has strong membranes which can limit the bioavailability of some nutrients and phytochemicals, novel methods and pretreatments are required with special emphasis on microbiological pretreatment methodologies. Pollen Chemistry & Biotechnology summarizes current knowledge of the chemical composition of pollen, its importance as a functional food ingredient and its health promoting properties. As Introduction part a short elaborate about botanical characteristics and data, including morphology and anatomy of pollen and bee preferences, is given. Important factors such as the botanical and geographical origin, best practices for preservation of nutritional composition, bioactivity and safety of pollen are covered in full. The nutritional profile of pollen based on data for minerals, lipids and nutrients is presented. A detailed phytochemical profile of pollen based on data for phenolics, flavonoids, carotenoids, vitamins, bioactive compounds is also covered. Areas for the improvement of the bioavailability of different nutrients and bioactive compounds of pollen via applied pretreatments are presented, as are best practices for adequate pollen collection, procession and storage in order to obtain safe products. At the end, an overview about pollen bioactivity as reason to use it as source of pharmaceuticals is made.

This book provides a Latino reading of John¿s prologue with special attention to how the themes of race, kinship, and the empire are part of the gospel¿s racial rhetoric. By drawing from the insights of Latinx texts and theology, this book reveals how the prologue provides a lens to read the entire gospel with a keen awareness of Jesus¿s engagement with people groups¿from his own family to the Roman authorities. The prologue participates in the gospel¿s racial rhetoric by shaping the reader¿s racial imagination even before a person enters the narrative. By doing so, Jesus¿s identity becomes constructed and defined through racial rhetoric since the opening verses of John¿s gospel.

This book takes an empirically grounded perspective on research in values, intimacy and sexuality, among other topics in psychology, to highlight the importance of searching for human subjectivity in its diversity, plurality and self-generativity. The author conducts an in-depth discussion on the methodological and epistemological issues enabling the study of subjectivity, and argues that in order to improve the contribution of psychology to human knowledge, a study of subjectivity must be at the forefront.This book presents a critical reflection of the author¿s decades-long research within psychology to argue for a significant paradigm shift in the conception and execution of psychological research: a shift to ¿second order psychology¿.

This book is the first to consider the roles, challenges and governance responses of secondary cities in southern Africa to changing circumstances. Among the challenges are governance under conditions of resource scarcity, managing informality, the effects and responses to climate change and the changing roles of the cities within the national space economy. It fills the gap in the literature on secondary cities with original case studies drawn from South Africa, Zimbabwe and Mozambique. The authors are all African scholars, working and living in the region with intimate knowledge of the settings they describe. The book is critical as it includes such regional case studies of different secondary cities in Southern Africa but also because of it¿s multidisciplinarity: it contains substantive and pertinent issues such as climate change, disaster management, local economic development, and basic services delivery. It considers diverse environments, yet with similar challenges that could provide useful policy and governance proposals for other cities.