Bøger udgivet af Humana Press Inc.

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

Discussing protocols in measuring components in the renin-angiotensin-aldosterone system (RAAS), this book also looks at methods to assess angiotensin peptides and the influence of RAAS components on mammalian diseases. In the Methods in Molecular Biology series, it lists the necessary materials in step-by-step, reproducible laboratory protocols.

The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling.

This thorough book covers the most recent proteomics techniques, databases, bioinformatics tools, and computational approaches that are used for the identification and functional annotation of proteins and their structure.

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases.

Edited by Dr. Mariusz Ratajczak, an internationally known specialist in adult stem cell biology, Adult Stem Cell Therapies: Alternatives to Plasticity is an important addition to the Stem Cell Biology and Regenerative Medicine series.

This volume contains a collection of writings from the leaders in the fields of Molecular Biology and Melanoma Research which will begin to tell the ever-expanding story of the most recent findings, discoveries, and potential of BRAF-directed targets in melanoma.

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes.

Section 1 covers glutamine structure and function, glutamine synthetase, glutamine binding protein, glutamine transport, glutamine-rich activation domains and transcription, glutamine transaminase and cell biochemistry.

Over the past 20 years, diagnostic tests for pediatric pulmonologists have revolutionized care of children afflicted with respiratory disorders.

This detailed volume focuses on the development of mycosis vaccines via the most common etiological agents of mycoses, such as Aspergillus and Candida species, followed by Cryptococcus, dimorphic fungi, and members of Mucoraceae family of fungi.

This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology.

In this volume, expert practitioners present a compilation of methods of functional data analysis (often referred to as "systems biology") and its applications in drug discovery, medicine, and basic disease research.

This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods.

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition.

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Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide.