Bøger udgivet af Humana Press Inc.

This book detailing various applications of fluorescent proteins delivered by viruses contains lab protocols that enable researchers to create rapid, readily reproducible results. It also highlights tips on troubleshooting and how to avoid pitfalls.

Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals.

Authorative and highly practical, Essential Urologic Laparoscopy: The Complete Clinical Guide, Second Edition provides a comprehensive guide to performing effective and cutting-edge urologic laparoscopy and is a must have for any urologist looking to create, maintain, or expand a successful practice in minimally invasive urologic surgery.

Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro.

Later chapters deal with statistical approaches to data analysis where the experimental objective is either to confirm the biology by identifying genomic regions associated to a trait or to use the data to make genomic predictions about a future phenotypic outcome (e.g.

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.

This Methods in Molecular Biology(TM) book is written on the premise that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. Offers materials lists, protocols and troubleshooting tips.

In recent years, technologies used to monitor and manipulate neuronal networks at single-cell resolution have expanded rapidly. This book examines the latest techniques in the field as well as highlighting current limitations and positing future developments.

Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I.

This Methods in Molecular Biology(TM) book offers techniques for studying the Permeability Barrier. Includes lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Robotic and Laparoscopic Reconstructive Surgery in Children and Adults is a unique volume focusing on the union between adult and pediatric urologic surgical techniques.

Recent discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. This book explores how the development of novel techniques has led to an increased understanding of lymphocyte trafficking.

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture.

In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells.

This up-to-date edition alternates scientific and clinical chapters that explain the basic science underlying neurological processes and then relates that science to the understanding of neurological disorders and their treatment.

Molecular Modeling of Proteins, Second Edition provides a theoretical background of various methods available and enables non-specialists to apply methods to their problems by including updated chapters and new material not covered in the first edition.

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner.

By providing a comprehensive overview of the current state of critical care ultrasonography, with content contributed by experts in the field, this text will serve as a valuable resource that will allow education in critical care ultrasound to keep pace with the rapid technological growth that has driven this vital area of critical care medicine.

"Additional material to this book can be downloaded from http://extras.springer.com" -- Title page vers

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis.

Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery.

This volume describes state-of-the-art protocols that serve as ¿recipes¿ for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology.

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases.

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike.