Bøger udgivet af Humana Press Inc.

The reader is then introduced to how mtDNA is transmitted through the oocyte and how stem cells can be used to study mitochondrial biogenesis and mtDNA replication and transcription in undifferentiated pluripotent and differentiating cells and how mitochondria adapt during this process.

Androgen Deficiency and Testosterone Replacement: Current Controversies and Strategies explores the difficulties around the diagnosis and treatment of androgen deficiency.

In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more.

This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene¿s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols.

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field.

This is the new and fully revised third edition of the well-received text that is the benchmark book in the field of nutrition and aging.

In this book, molecular defects in cell death signaling that characterize cancer cells, including dysregulation of cell death due to overexpression/hyperactivation of oncoproteins, as well as the loss of tumor suppressor proteins will be described.

Gastroesophageal Reflux and the Lung provides a comprehensive review of current knowledge concerning normal deglutition and foregut digestive processes and examines how abnormalities of swallowing or excessive/abnormal GER can lead to respiratory tract dysfunction and lung disease.

Cytokines in the Genesis and Treatment of Cancer provides a comprehensive picture of the dual role of host responses in promoting and inhibiting tumor progression. This volume represents an important investigation into the emerging intersection of cancer biology and cancer immunology.

Cancer Neurology in Clinical Practice: Neurologic Complications of Cancer and its Treatment, Second Edition provides clinicians from various backgrounds and levels of training with a reference to help focus the differential diagnosis, treatment strategy, and management plan for the cancer patient with neurologic symptoms and findings.

Cellular Respiration and Carcinogenesis presents leading experts in the field as it informs the reader about both basic and recent research in the field of cellular respiration and the effects of its dysfunction, alteration or attenuation on the development of cancer.

Juvenile homicide and fatal maltreatment remain serious and pervasive problems in the developed world and especially in the United States, where in 2005 some 1,500 children died from neglect and physical abuse.

From its chapters on the history and core principles of clinical forensic medicine, right up to the text's discussion of substance misuse and deaths in custody, this volume remains the gold standard source for health professionals whose working lives bring them into contact with those in the law enforcement arena.

It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques.

This is a comprehensive volume about the evolving new field AllergoOncology. The book, the first of its class, attempts to explore step by step the interesting questions emerging from this novel science field.

It covers the tried and true topics of fingerprints, trace evidence, chemistry, biology, explosives and arson, forensic anthropology, forensic pathology, forensic documents, firearms and toolmarks.

Experimental and Applied Immunotherapy offers a roadmap to the creation of immunotherapies, which have prominent cancer-fighting potential. Readers will find discussions of T cell and non-T cell therapy, monoclonal antibody therapy, dendritic cell-based cancer vaccines, and more.

Footwear and pedal evidence have become valuable in forensic investigations. This is the first textbook ever dedicated to forensic podiatry. It is an international compilation of current practices authored by pioneers in the field. It includes case studies.

Over the last 20 years, pediatric otolaryngology has become a recognized subspecialty within otolaryngology head and neck surgery.

On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials.

This volume is a valuable and timely resource for a broad audience with interests in basic and translational cancer biology, cancer drug development, as well as in the practice of personalized oncology.

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced.

"Published In: United States, 07 December 2016 " --Distributor's website.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.

This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The knowledge of host-related predisposing factors and stratified treatment options facilitating timely onset of adequate antifungal therapy are critical for successful clinical management and outcome of invasive fungal disease (IFD), requiring not only rapid diagnosis of a fungal infection and identification of the causative species, but also assessment of pathogen/host factors related to pathogenicity, susceptibility, and response to treatment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tipson troubleshooting and avoiding known pitfalls.Authoritative and practical, Human Fungal Pathogen Identification: Methods and Protocols serves as an ideal reference for researchers investigating the ever-growing worldwide healthcare problems involving fungal infections.

This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells.