Bøger udgivet af Cold Spring Harbor Laboratory Press,U.S.

Based on Sydney Brenner's personal recollections, with contributions and correspondence from his close friends and colleagues, this book tells the lively story, not only of Brenner himself, but of what came to be known as the golden age of biology.

This volume reviews our understanding of two RNA worlds: the primordial RNA world before DNA, in which RNA was both information store and biocatalyst; and the contemporary RNA world, in which mRNA, tRNA, rRNA, siRNA, miRNA, and a host of other RNAs operate.

Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.

The behavior of lymphocytes in the immune system depends on encounters with antigens. These bind to immunoreceptors on the surface of T-cells and B-cells, activating a variety of signal transduction pathways that control cell survival, proliferation, differentiation, and effector functions. Written and edited by experts in the field, this volume is essential reading for systems biologists as well as all immunologists and cell biologists interested in understanding how lymphocytes function.

A manual containing new, established, and evolving methods for real-time studies of how living cells and organisms work. Appropriate for working scientists from graduate level up to principal investigator.

Experts examine the mechanisms by which cells polarize, divide asymmetrically, and produce asymmetric structures, providing examples from bacteria, yeast, plants, invertebrates, and mammals. Discussion include the molecular basis of polarization, mechanisms, and more.

Many human diseases--including Alzheimer's disease, schizophrenia, cancer, and cardiovascular disease--show complex inheritance that requires sophisticated analysis. Genetics of Complex Human Diseases: A Laboratory Manual brings together the tools that geneticists use to find disease genes with the genetic concepts and statistical theories that underpin these research approaches. Topics covered include basic genetics and Mendelian inheritance, statistical methods, genetic epidemiology, linkage studies, transmission disequilibrium test analysis, variance components analysis, genome-wide association studies, copy-number variation, methods for high-throughput genotyping, the complexity of RNA editing, and genetic computer programs. The book's chapters, written by leading investigators in the field, blend practical information and reviews of each topic, providing both the how and the why of complex disease analysis. Genetics of Complex Human Diseases is an important guide for anyone with an interest in human genetics or who uses genetic techniques in the study of diseases with complex inheritance.

Signaling by diffusible morphogens, such as Hedgehog, Wingless, TGF-, and various growth factors, is vital for developmental patterning, ensuring that distinct differentiated cell types appear in the right place and at the right time in forming tissues. The expert contributors to this volume discuss the roles of morphogen gradients in organisms from yeast to humans. They examine the regulation of morphogen synthesis, trafficking, and diffusion, as well as the complex webs of signaling mechanisms and transcriptional responses in recipient cells. The book is a vital reference for developmental biologists and cell biologists wishing to know how cell fate is determined during embryogenesis.

Comprised of review articles reprinted from the tenth anniversary issue of the journal "Genome Research" that capture the status of genetic research in a collection of model species, this volume serves as a valuable reference and teaching tool for established scientists in the genomics field and as a comprehensive overview for those with more general interests in biology.

This is the first compendium of protocols specifically geared towards genetic variation studies. It includes detailed step-by-step experimental protocols that cover the complete spectrum of genetic variation in humans and model organisms, along with advice on study design and analyzing data.

This is the first book to provide a detailed and thoroughly up-to-date review of the complexity of DNA replication in eukaryotic cells. It is organized into three parts: Concepts, a distillation of underlying principles; Enzymes, a description of each protein class involved; and Systems, a review of events over a wide range of organisms. The book is therefore invaluable for teachers who want a current survey of a topic central to the biology syllabus: investigators of replication who will appreciate a remarkably concise, central source of knowledge in their specialty; and scientists studying other biological functions on which DNA replication has an impact.

For James D. Watson, the year 2003 was momentous: The 50th anniversary of the discovery, with Francis Crick, of the DNA double helix; the 35th anniversary of the publication of his best-selling memoir of the discovery, The Double Helix; the 35th anniversary of his appointment as Director of Cold Spring Harbor Laboratory, an institution he molded into a research and education center of international renown and prestige: and the year in which the sequencing of the human genome was completed, a project of unprecedented international effort and coordination that Watson got off the ground and sustained during its first, critical years. This book gives voice to 43 of these individuals--people of distinction who have worked with Watson as a scientist, educator, author, administrator, and government official. Their essays cover much of his scientific life and, taken together, create a portrait of a complex man whose originality and force of will have produced extraordinary achievements.

Highly illustrated and based heavily on early 20th century original sources, the book traces the roots of genetics in breeding analysis and studies of cytology, evolution, and reproductive biology that began in Europe but were synthesized in the United States through new Ph.D. programs and expanded academic funding. Readable and original, this narrative will interest historians and science educators as well as today's practitioners of genetics.

Ageless Quest is a personal, sometimes controversial, account of the pursuit of a genetic 'cure' for aging by an expert in the field. The author is the Novartis Professor of Biology at the Massachusetts Institute of Technology. Aging has always been regarded as a highly complex process with many degenerative changes leading to the cessation of life. But recent research has identified a relatively simple mechanism that governs the pace of aging. Lenny Guarente's Ageless Quest is a scientific detective story for the baby boom generation. It offers an insider's view of an area of potentially astonishing high reward--and equally high risk.

"99% of mouse protein-coding genes have an equivalent homolog in the human genome, despite the striking differences in appearance between mouse and man. This remarkable genetic similarity, together with our ability to finely engineer the murine genome, has made the mouse the ideal animal in which to model and analyze human biology and disease. This