Bøger i Neuromethods serien

This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods.

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system.

Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity.

The chapters in this book discuss the use of fluorescent metal probes, synchrotron-based X-ray microscopy, ICP-MS, laser ablation-ICP-MS, laser-based tissue microdissection, MRI image analysis, fractionation of cell tissue samples for metal analysis, and metal treatment of cells.

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience.

In recent years, technologies used to monitor and manipulate neuronal networks at single-cell resolution have expanded rapidly. This book examines the latest techniques in the field as well as highlighting current limitations and positing future developments.

Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals.

Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience.

This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.

This volume provides readers with an introduction to all the methods and major approaches now being used to study the structural and functional development of the brain.

Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples;

This volume provides comprehensive information and detailed laboratory protocols used in the study of prion diseases. Chapters also provide readers with detailed information about kuru, the clinical description of prion diseases, and the detection of prion protein and biomarkers.

As fMRI technology has provided invaluable insights into the mechanisms through which the human brain works in healthy individuals and in patients with different neurological and psychiatric conditions, the study of brain function and even the monitoring of the effects of treatment have become more effective and efficient.

Later chapters describe how these paradigms are used to model the progression of drug addiction, providing insight into the clinical symptomatology of addiction from acquisition of drug use through compulsive drug taking to withdrawal and relapse.

Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi).

In view of the numerous failures of clinical trials aimed at improving stroke therapy, the role and potential benefit of experimentally modeling focal cerebral ischemia in rodents has been debated.

Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fields from psychopharmacology, neurobiology, and physiology in animals and also humans.

The chapters discuss the use and interpretation of video-aided quantification of zebrafish behaviors, describe novel assays commonly used to quantify emotionality, learning, memory, and social behaviors in zebrafish, and instruct how to quantify endocrine responses and correlate them with behavior.

Although the available models, whether at the cellular, tissue, or animal level, do not exactly represent the biology of human brain tumors, animal models can offer significant insights into these tumors, providing a better understanding of biological mechanisms underlying tumor generation, growth, angiogenesis, invasion, and metastasis.